Write the correct sequence of events performed in DNA fingerprinting technique
A. Isolation of DNA
B. Separation of DNA fragments by electrophoresis
C. Digestion of DNA using restriction endonucleases.
D. Transfer of separated DNA fragments to synthetic membrane.
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 1
The correct answer is Option (1) → A, C, B, D
A. Isolation of DNA — first.
C. Digestion of DNA using restriction endonucleases. — second. The DNA is cut into fragments.
B. Separation of DNA fragments by electrophoresis — third. The fragments are separated by size.
D. Transfer of separated DNA fragments to synthetic membrane. — fourth. This blotting is followed by hybridisation with a VNTR probe and autoradiography.
NCERT Extract:
DNA Fingerprinting – “… The technique, as used earlier, involved Southern blot hybridisation using radiolabelled VNTR as a probe. It included
(i) isolation of DNA,
(ii) digestion of DNA by restriction endonucleases, (iii) separation of DNA fragments by electrophoresis,
(iv) transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon,
(v) hybridisation using labelled VNTR probe, and
(vi) detection of hybridised DNA fragments by autoradiography.”