Recombinant DNA technology involves the following steps in a specific sequence.
(A) Culture of the host cell
(B) Extraction of desired product
(C) Isolation of desired DNA fragments
(D) Transformation of the recombinant DNA into the host cell
Arrange these sequences correctly and choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → (C), (D), (A), (B)
(C) Isolation of desired DNA fragments — first. The DNA is taken out of the cells and cut with restriction enzymes, and the fragment with the gene of interest is picked out.
(D) Transformation of the recombinant DNA into the host cell — second. The fragment is joined to a vector by ligase, and the recombinant DNA is put into a competent host, usually a bacterium.
(A) Culture of the host cell — third. The transformed host is grown on a large scale, often in a bioreactor, so that the gene makes its product.
(B) Extraction of desired product — last. The product is separated and purified; this is downstream processing.
NCERT Extract:
Processes of Recombinant DNA Technology – …
