Choose the correct sequence of events involved in the process of RNA interference.
(A) dsRNA silences the mRNAs produced by nematode
(B) Using Agrobacterium vector nematode specific genes are introduced into the host plant
(C) Two strands of RNA being complementary to each other form dsRNA
(D) DNA is introduced in such a way that it produces both sense and antisense RNA in the host
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 3
The correct answer is Option (3) → (B), (D), (C), (A)
In RNA interference against Meloidogyne incognitia, nematode-specific genes are first introduced into the host plant using an Agrobacterium vector (B). The DNA is introduced so that it produces both sense and antisense RNA (D). The two complementary RNAs form dsRNA (C), which silences the specific mRNA of the nematode (A). The order is (B), (D), (C), (A).
NCERT Extract:
Biotechnological Applications in Agriculture – “… Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant (Figure 10.2). The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNA’s being complementary to each other formed a double stranded (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode.”