Arrange the given steps of recombinant DNA technology in correct sequence.
A. Insertion of recombinant DNA into the host
B. Isolation of the genetic material
C. Amplification of gene of interest
D. Obtain the foreign gene product
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → B, C, A, D
(B) Isolation of the genetic material — comes first. The DNA has to be separated from the cell before anything can be done to it. The cells are treated with enzymes, and the DNA is then purified.
(C) Amplification of gene of interest — comes second. The gene of interest is copied many times by PCR, so there is enough of it to be joined to a vector.
(A) Insertion of recombinant DNA into the host — comes third. Only after the gene is joined to a vector can the recombinant DNA be put into a host cell.
(D) Obtain the foreign gene product — comes last. The host cells make the protein only after they carry the recombinant DNA, and the product is then taken out and purified.