Which one of the following enzymes is not used to release the DNA from the organisms?
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → Permease
DNA is enclosed inside the cell and its membranes. To cut it with restriction enzymes, the cell must first be broken open. Each type of cell needs the enzyme that dissolves its own wall.
(1) Lysozyme — Used to release DNA from cells. It breaks the cell wall of bacteria.
(2) Permease — Not used to release DNA from cells. It is a membrane protein that carries a substance (such as lactose in the lac operon) into the cell. It does not break a cell wall.
(3) Chitinase — Used to release DNA from cells. It breaks the chitin wall of fungi.
(4) Cellulase — Used to release DNA from cells. It breaks the cellulose wall of plant cells.
NCERT Extract:
Isolation of the Genetic Material (DNA) – “… Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).”