A method in which cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA is known as:
Answer & explanation
Correct answer: option 3
The correct answer is Option (3) → Biolistics
Biolistics, also called the gene gun, is a method used for plants.
(1) Cloning — Incorrect. This is making identical copies of a gene or an organism. It is not a way of putting DNA into a cell.
(2) Micro injection — Incorrect. Here DNA is injected directly into the nucleus of an animal cell with a fine needle. No particles are fired.
(3) Biolistics — Correct. This is the particle-bombardment method.
(4) Amplification — Incorrect. This is making many copies of a DNA piece, as in PCR.
NCERT Extract:
Cloning Vectors – “… Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 420C (heat shock), and then putting them back on ice. This enables the bacteria to take up the recombinant DNA. This is not the only way to introduce alien DNA into host cells. In a method known as micro-injection, recombinant DNA is directly injected into the nucleus of an animal cell. In another method, suitable for plants, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun.”