Practicing Success
Practicing Success
CUET Preparation Today
What is the purpose of using DNA ligase in the construction of recombinant DNA? |
To cut DNA at specific locations To join the ends of cut DNA molecules To deliver an alien piece of DNA into the host organism To multiply copies of the antibiotic resistance gene |
To join the ends of cut DNA molecules |
b) To join the ends of cut DNA molecules The purpose of using DNA ligase in the construction of recombinant DNA is to join the ends of cut DNA molecules. After a specific piece of DNA is cut using restriction enzymes, DNA ligase is used to catalyze the formation of phosphodiester bonds between the sugar-phosphate backbones of the two DNA fragments. This process creates a new DNA molecule by linking the two fragments together, resulting in a recombinant DNA molecule. DNA ligase plays a crucial role in the genetic engineering process as it allows researchers to combine DNA from different sources to create new genetic combinations with desired traits or characteristics.
|
