Which one is responsible to cause inbreeding depression?

Continued self-pollination.

The correct answer is Option (1) – Continued self-pollination.

Inbreeding depression occurs when there is repeated self-pollination or mating between closely related individuals. This leads to a reduction in genetic diversity and an increase in the likelihood of harmful recessive traits being expressed, which can result in weaker or less viable offspring.

"No pollination" does not cause inbreeding depression When there's no pollination at all, reproduction simply doesn't occur, leading to a lack of seed production. This results in no offspring being produced, rather than offspring that suffer from reduced fitness due to inbreeding. Therefore, the issue in "no pollination" is infertility, not inbreeding depression.

While continued cross pollination will increase the genetic diversity of the population, which can help to reduce the effects of inbreeding depression.

Therefore, the answer is continued self-pollination.