Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion of DNA. In this technique, the movement of a DNA does not depend on -
Answer & explanation
Correct answer: option 1
The correct answer is Option (1) → Color of DNA
Option (1) Color of DNA — The movement of DNA does not depend on it. DNA fragments have no colour of their own. They cannot be seen in visible light at all. They are stained with ethidium bromide and seen under UV light only after the run, so colour plays no part in how far they move.
Option (2) Large size — The movement of DNA depends on it. The agarose gel acts as a sieve. Large fragments are held back and stay closer to the well.
Option (3) Small size — The movement of DNA depends on it. Small fragments slip through the gel more easily, so they travel farther in the same time.
Option (4) Charge on DNA — The movement of DNA depends on it. DNA is negatively charged, so in the electric field it moves towards the anode. Without this charge it would not move at all.
NCERT Extract:
Restriction Enzymes – …
