Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Read the following passage carefully.

Restriction enzyme

Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. The word palindromes refer to group of letters that forms the same words when read both forward and backward. Restriction endonucleases are used in genetic engineering as molecular scissors.

Overhanging stretches on each strand is called:

Options:

Hydrogen bond

Sticky end

Ligase

Palindrome

Correct Answer:

Sticky end

Explanation:

The correct answer is Option (2) → Sticky end    

Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. Restriction endonucleases are used in genetic engineering to form ‘recombinant’ molecules of DNA, which are composed of DNA from different sources/genomes. When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of ‘sticky-ends’ and, these can be joined together (end-to-end) using DNA ligases.