Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Which enzymes would you use to disrupt the cell membranes in the following cells?
i)Bacteria
ii)Fungi
iii)Plant cells

Options:

i- Protease
ii- Cellulase
iii- Chitinase

i- Lysozyme
ii- Chitinase
iii- Cellulase

i-Chitinase
ii-Lipase
iii- Cellulase

i- Lysozyme
ii-Cellulase
iii-Lipase

Correct Answer:

i- Lysozyme
ii- Chitinase
iii- Cellulase

Explanation:

Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).