In which among the following method sperm is directly injecting into ovum in assisted reproductive technology ?

ICSI

Option 1 is correct . ICSI

In vitro fertilisation (IVF–fertilisation outside the body in almost similar conditions as that in the body) followed by embryo transfer (ET) is one of such methods. In this method, popularly known as test tube baby programme, ova from the wife/donor (female) and sperms from the husband/donor (male) are collected and are induced to form zygote under simulated conditions in the laboratory.

The zygote or early embryos (with upto 8 blastomeres) could then be transferred into the fallopian tube (ZIFT–zygote intra fallopian transfer) and embryos with more than 8 blastomeres, into the uterus (IUT – intra uterine transfer), to complete its further development.

Intra cytoplasmic sperm injection (ICSI) is another specialised procedure to form an embryo in the laboratory in which a sperm is directly injected into the ovum.