Arrange the following events in the order of their occurrence in tobacco plants to prevent infestation of nematode (Meloidegyne incognitia), based on the process of RNA interference.
(A) Specific mRNA of the nematode is silenced
(B) DNA produced sense and anti-sense RNA
(C) Nematode specific genes were introduced into the host plant
(D) dsRNA is formed and RNAi is initiated
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 4
The correct answer is Option (4) → (C), (B), (D), (A)
(C) Nematode specific genes were introduced into the host plant — first. Using Agrobacterium vectors, genes of the nematode were put into tobacco.
(B) DNA produced sense and anti-sense RNA — second. The introduced DNA was such that it made both sense and anti-sense RNA in the host cells.
(D) dsRNA is formed and RNAi is initiated — third. The two RNA strands, being complementary, pair to form dsRNA, which starts RNA interference.
(A) Specific mRNA of the nematode is silenced — last. The dsRNA binds the nematode’s matching mRNA and stops its translation, so the parasite cannot survive in the plant.
NCERT Extract:
Biotechnological Applications in Agriculture – …
