Practicing Success
How many cycles in a PCR will be required for amplifying the genetic material to about one billion times? |
10 cycles 15 cycles 30 cycles 35 cycles |
30 cycles |
The correct answer is Option (3)- 30 cycles PCR (Polymerase Chain Reaction) is a technique that is used to make millions of copies of a sample DNA very rapidly. After the completion of each cycle, two copies of DNA samples are produced. So, From 1 DNA molecule we get 2n molecules after n number of cycles. If the process of replication of DNA is repeated many times, the segment of DNA can be amplified to approximately billion times, i.e., 1 billion copies are made at the end of 30 PCR cycles. It is possible to generate 2n DNA molecules after ‘n’ number of cycles. Therefore 2n = 230 =1,073,741,824 PCR is indeed a technique that can exponentially amplify a single copy of a specific DNA segment, generating thousands to millions of copies of that segment. The concept of 2n copies being produced after each cycle, where n is the number of cycles, is fundamental to understanding the power of PCR in DNA amplification.
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