Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

The given figure is the diagrammatic representation of the vector pBR322.Which one of the following option correctly identifies its components ?

Options:

Ori-original restriction enzymes.

Rop-reduced osmotic pressure.

Hind III, Eco RI -selectable markers .

ampR ,tetR  _ antibiotic resistance genes .  

Correct Answer:

ampR ,tetR  _ antibiotic resistance genes .  

Explanation:

The correct answer is Option (4) –ampR ,tetR  _ antibiotic resistance genes . 

E. coli cloning vector pBR322 showing restriction sites (Hind III, EcoR I, BamH I, Sal I, Pvu II, Pst I, Cla I), ori and antibiotic resistance genes (ampR and tetR). rop codes for the proteins involved in the replication of the plasmid.