Which of the following technique is used for separating DNA fragments?

Gel electrophoresis

The technique used for separating DNA fragments is Gel Electrophoresis.

Gel electrophoresis is a laboratory technique used to separate and analyze DNA, RNA, or proteins based on their size and charge. In this method, DNA fragments are loaded onto a gel matrix and subjected to an electric field. The negatively charged DNA molecules move towards the positive electrode, and the smaller fragments move faster through the gel matrix than the larger ones. As a result, the DNA fragments get separated based on their sizes, allowing for their visualization and analysis.