For transformation, the DNA-coated micro-particles bombarded with a gene gun are made of which of the following metals?
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → Gold or tungsten
Recombinant DNA can be put into host cells in several ways. In a method suited to plants, called biolistics or the gene gun, the cells are bombarded with very fast micro-particles of gold or tungsten that are coated with DNA. The particles carry the DNA into the cells. Other methods are micro-injection of DNA into the nucleus of an animal cell, and the use of ‘disarmed pathogen’ vectors.
NCERT Extract:
Competent Host (For Transformation with Recombinant DNA) – “… This is not the only way to introduce alien DNA into host cells. In a method known as micro-injection, recombinant DNA is directly injected into the nucleus of an animal cell. In another method, suitable for plants, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun.”