RNA polymerase III is responsible for transcription of -

The correct answer is Option (3) → tRNA, 5srRNA, and snRNAs

Bacteria have one RNA polymerase for all their RNAs. Eukaryotes have at least three in the nucleus, each with its own work.

Option (1) rRNAs — Incorrect. The large rRNAs (28S, 18S and 5.8S) are made by RNA polymerase I. Only the small 5S rRNA is left to polymerase III.

Option (2) hnRNA — Incorrect. hnRNA, the precursor of mRNA, is made by RNA polymerase II. It then gets a cap and a tail, and its introns are removed by splicing.

Option (3) tRNA, 5srRNA, and snRNAs — Correct. These are the small RNAs, and RNA polymerase III makes all three.

Option (4) ds RNA — Incorrect. Double-stranded RNA is not a product of any of the three polymerases. It appears in RNA interference, where it silences a matching mRNA.

Note: A similar question was also asked in CUET (UG) 2025 on 20-05-2025 (Slot 2), and in CUET (UG) 2023 on 15-06-2023 (Slot 1).

NCERT Extract:
Types of RNA and the Process of Transcription – “… (i) There are at least three RNA polymerases in the nucleus (in addition to the RNA polymerase found in the organelles). There is a clear cut division of labour. The RNA polymerase I transcribes rRNAs (28S, 18S, and 5.8S), whereas the RNA polymerase III is responsible for transcription of tRNA, 5srRNA, and snRNAs (small nuclear RNAs). The RNA polymerase II transcribes precursor of mRNA, the heterogeneous nuclear RNA (hnRNA).”