Each seminiferous tubule of the human testis is lined on its inside b…

The correct answer is Option (2) → Male germ cells (spermatogonia) and Sertoli cells

Each testis has about 250 lobules, and each lobule holds one to three highly coiled seminiferous tubules in which sperms are made. The inside of each tubule is lined by two types of cells: male germ cells (spermatogonia), which divide by meiosis and finally form sperms, and Sertoli cells, which nourish the germ cells. The spaces outside the tubules hold other cells.

Option (1) Leydig cells and Sertoli cells — Incorrect. Leydig cells lie outside the tubules, in the interstitial spaces, where they make androgens. Only the Sertoli cells in this pair line the tubule.

Option (2) Male germ cells (spermatogonia) and Sertoli cells — Correct. The tubule is lined on its inside by spermatogonia and Sertoli cells. The spermatogonia give rise to sperms, and the Sertoli cells feed them.

Option (3) Male germ cells (spermatogonia) and Leydig cells — Incorrect. Spermatogonia do line the tubule, but Leydig cells do not. They are interstitial cells found in the spaces between the tubules.

Option (4) Sertoli cells and immunocompetent cells — Incorrect. Immunologically competent cells are found in the interstitial spaces, along with blood vessels and Leydig cells, not in the lining of the tubule.

NCERT Extract:
The Male Reproductive System – “… Each lobule contains one to three highly coiled seminiferous tubules in which sperms are produced. Each seminiferous tubule is lined on its inside by two types of cells called male germ cells (spermatogonia) and Sertoli cells (Figure 2.2). The male germ cells undergo meiotic divisions finally leading to sperm formation, while Sertoli cells provide nutrition to the germ cells. The regions outside the seminiferous tubules called interstitial spaces, contain small blood vessels and interstitial cells or Leydig cells (Figure 2.2). Leydig cells synthesise and secrete testicular hormones called androgens.”