Which one of the following is not a process of DNA recombinant technology?
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → RNA interference (RNAi)
Option (1) Isolation of DNA — A process of recombinant DNA technology. It is the first step. The cell is broken open with enzymes, and the pure DNA is separated from RNA and proteins.
Option (2) RNA interference (RNAi) — Not a process of recombinant DNA technology. RNAi is a method of cellular defence in eukaryotes: a double-stranded RNA silences a matching mRNA. It is an application of biotechnology, used to make nematode-resistant tobacco plants, not a step in making recombinant DNA.
Option (3) Introduction of Restriction endonucleases — A process of recombinant DNA technology. Restriction endonucleases are added to cut the DNA at specific sites, both the source DNA and the vector.
Option (4) Culturing the host cells in a medium at a large scale — A process of recombinant DNA technology. The host cells carrying the recombinant DNA are grown in bioreactors, so that the desired product is made in large amounts.
NCERT Extract:
Processes of Recombinant DNA Technology – “… Recombinant DNA technology involves several steps in specific sequence such as isolation of DNA, fragmentation of DNA by restriction endonucleases, isolation of a desired DNA fragment, ligation of the DNA fragment into a vector, transferring the recombinant DNA into the host, culturing the host cells in a medium at large scale and extraction of the desired product.”
Biotechnological Applications in Agriculture – “… A novel strategy was adopted to prevent this infestation which was based on the process of RNA interference (RNAi).”