Which one of the following is not a process of DNA recombinant technology?

RNA interference (RNAi)

The correct answer is Option (2) → RNA interference (RNAi)

The processes involved in recombinant DNA technology include:

• Isolation of DNA: Extracting DNA from cells to work with the gene of interest.
• Introduction of Restriction endonucleases: Using restriction enzymes to cut DNA at specific sites for gene insertion.
• Culturing the host cells in a medium at a large scale: Growing transformed host cells on a large scale to produce the desired gene product.

RNA interference (RNAi): Several nematodes parasitise a wide variety of plants and animals including human beings. A nematode Meloidegyne incognitia infects the roots of tobacco plants and causes a great reduction in yield. A novel strategy was adopted to prevent this infestation which was based on the process of RNA interference (RNAi . RNA interference (RNAi) takes place in all eukaryotic organisms as a method of cellular defense. This method involves silencing of a specific mRNA due to the complementary dsRNA molecule that binds to and prevents translation of the mRNA. The source of this complementary RNA could be from an infection by viruses having RNA genomes that replicate via an RNA intermediate.

Therefore, the correct answer is: RNA interference (RNAi)