The construction of the first recombinant DNA emerged from linking a gene encoding antibiotic resistance with a native plasmid of ____________.
Answer & explanation
Correct answer: option 4
The correct answer is Option (4) → Salmonella typhimurium
The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid (autonomously replicating circular extra-chromosomal DNA) of Salmonella typhimurium. Stanley Cohen and Herbert Boyer accomplished this in 1972 by isolating the antibiotic resistance gene by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance.