Arrange the given steps of DNA fingerprinting in sequence.
(A) Transferring of separated DNA fragments on nitrocellulose membrane
(B) Isolation of DNA and digestion by restriction endonuclease
(C) Hybridisation using VNTR Probe
(D) Separation of isolated DNA fragments by Gel Electrophoresis
Choose the correct answer from the options given below :
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → (B), (D), (A), (C)
In DNA fingerprinting, DNA is isolated and cut by restriction enzymes (B), the fragments are separated by gel electrophoresis (D), transferred to a nitrocellulose or nylon membrane (A), and hybridised with a VNTR probe (C).
Note: A similar question was also asked in CUET (UG) 2026 on 29-05-2026 (Slot 2).
NCERT Extract:
DNA Fingerprinting – “… The technique, as used earlier, involved Southern blot hybridisation using radiolabelled VNTR as a probe. It included
(i) isolation of DNA,
(ii) digestion of DNA by restriction endonucleases, (iii) separation of DNA fragments by electrophoresis,
(iv) transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon,
(v) hybridisation using labelled VNTR probe, and
(vi) detection of hybridised DNA fragments by autoradiography.”