Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Read the following passage and answer the questions that follow:-
Deeksha lives in a society where a robbery occurred last night. Robbers came into the flat and murdered the old lady residing there. Police came and restricted the entry into the flat. They took samples from the room, where the dead body was found. While examining, they found that there is some blood and tissue in the nails of an old lady. According to their observation, police filtered out their inspection to three suspects viz. servant, cook, and milkman. Finally, after two days of robbery, police caught the criminal. It was the old lady’s cook. Deeksha was amazed to see how quickly the police completed and shut the case. She asked the inspector how they did it? The policeman told her that it become possible due to the sample collected from the victim, which lead them to the criminal. The sample taken from nail scraping was amplified using PCR and then tested.

Given below are the steps of the polymerase chain reaction

Select the option that correctly mentions the sequence in which they occur.

Options:

(ii) → (iii) → (i)

 

(i) → (ii) → (iii)

 

(iii) → (i) → (ii)

 

(ii) → (i) → (iii)

 

Correct Answer:

(ii) → (iii) → (i)

 

Explanation:

The correct answer is Option (4) -(ii) → (iii) → (i)

Denaturation  → Annealing  → Extension are the three steps of PCR.

The PCR involves three major cyclic reactions:

Denaturation

Annealing

Elongation