Which one of the following step is not involved in the genetic modification of an organism?
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → Cutting of DNA with DNA ligase
There are three basic steps in genetically modifying an organism: identifying the DNA with the desirable genes, introducing it into the host, and maintaining it in the host so that it passes to the progeny.
Option (1) Identification of DNA with desirable genes — A step of genetic modification. It is the first step.
Option (2) Cutting of DNA with DNA ligase — Not a step of genetic modification. Ligase joins DNA; it does not cut it. Cutting is done by restriction enzymes.
Option (3) Introduction of the identified DNA into the host — A step of genetic modification. It is the second step.
Option (4) Maintenance of introduced DNA in the host and transfer of the DNA to its progeny. — A step of genetic modification. It is the third step.