Which one of the following is not the tool of recombinant DNA technology?
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → Lipase
Recombinant DNA technology can be done only with the right tools: restriction enzymes, polymerase enzymes, ligases, vectors and the host organism.
(1) DNA polymerase — A tool of recombinant DNA technology. A polymerase makes new DNA, as in PCR.
(2) Lipase — Not a tool of recombinant DNA technology. Lipase breaks down fats. It does not cut, join or copy DNA.
(3) Ligase — A tool of recombinant DNA technology. It joins the cut DNA pieces together.
(4) Restriction enzyme — A tool of recombinant DNA technology. It cuts DNA at specific sites.
Note: A similar question was also asked in CUET (UG) 2026 on 30-05-2026 (Slot 2).
NCERT Extract:
Tools of Recombinant DNA Technology – …
