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Observation of 'Rhumann's purple' is a confirmatory test for the presence of :
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Reducing sugar Cupric ion Protein Starch |
Protein |
Ninhydrin (2,2-dihydroxyindane-1,3-dione) is a chemical used to detect ammonia or primary and secondary amines. When reacting with these free amines (proteins), a deep blue or purple colour known as Ruhemann’s purple is produced.
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