The methods employed to introduce recombinant DNA into host cell is/are
A. Micro-injection
B. Ligation
C. Cation treatment and heat shock
D. Gene gun
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 3
The correct answer is Option (3) → A, C and D only
A. Micro-injection — True.
B. Ligation — False. Ligation joins DNA fragments; it does not introduce DNA into the host.
C. Cation treatment and heat shock — True.
D. Gene gun — True.
NCERT Extract:
Cloning Vectors – “… Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 420C (heat shock), and then putting them back on ice. This enables the bacteria to take up the recombinant DNA. This is not the only way to introduce alien DNA into host cells. In a method known as micro-injection, recombinant DNA is directly injected into the nucleus of an animal cell. In another method, suitable for plants, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun.”