Which of these is not a method used for introduction of plasmid into host cells?
Answer & explanation
Correct answer: option 1
The correct answer is Option (1) → Polymerase chain reaction
Polymerase chain reaction (PCR) is not a method used for the introduction of a plasmid into host cells. PCR is a laboratory technique used to amplify a specific DNA sequence by producing multiple copies of that DNA segment. It does not involve the direct introduction of plasmids into host cells.
On the other hand:
- Gene gun is a biolistic method used to deliver DNA or plasmids into cells by high-speed microprojectiles.
- Microinjection involves physically injecting genetic material (such as plasmids) into cells using a fine micropipette.