Leydig cells are present in -

The correct answer is Option (2) → Interstitial spaces

(1) Epididymis — Incorrect. The epididymis is a coiled duct along the back of each testis. It stores sperms for a short time and carries them to the vas deferens. It has no hormone-making cells.

(2) Interstitial spaces — Correct. The regions of the testis lying outside the seminiferous tubules are called interstitial spaces. They hold small blood vessels and the interstitial cells or Leydig cells. Leydig cells make and release the male hormones called androgens.

(3) Seminiferous tubule — Incorrect. Inside the seminiferous tubules there are two kinds of cells: the male germ cells (spermatogonia), which form sperms, and the Sertoli cells, which nourish them. Leydig cells lie outside these tubules.

(4) Rete testis — Incorrect. The rete testis is a network of fine tubes. The seminiferous tubules open into it, and it passes the sperms on to the vasa efferentia. It carries sperms; it does not make hormones.

NCERT Extract:
The Male Reproductive System – “… Each seminiferous tubule is lined on its inside by two types of cells called male germ cells (spermatogonia) and Sertoli cells (Figure 2.2 ). The male germ cells undergo meiotic divisions finally leading to sperm formation, while Sertoli cells provide nutrition to the germ cells. The regions outside the seminiferous tubules called interstitial spaces, contain small blood vessels and interstitial cells or Leydig cells (Figure 2.2). Leydig cells synthesise and secrete testicular hormones called androgens. Other immunologically competent cells are also present.”