The correct sequence of steps involved in Southern blot hybridization are:
(A) Isolation of DNA followed by digestion by restriction endonucleases
(B) Separation of DNA fragments by electrophoresis
(C) Blotting of separated DNA fragments
(D) Detection of hybridized DNA fragments by autoradiography
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → (A), (B), (C), (D)
(A) Isolation of DNA followed by digestion by restriction endonucleases — this comes first. The DNA is taken out of the cells and cut into pieces.
(B) Separation of DNA fragments by electrophoresis — this comes second. The cut pieces are sorted by size in a gel.
(C) Blotting of separated DNA fragments — this comes third. The sorted fragments are transferred from the gel to a membrane, such as nitrocellulose or nylon.
(D) Detection of hybridized DNA fragments by autoradiography — this comes last. A labelled probe sticks to its matching fragments on the membrane, and these are seen on a photographic film.
NCERT Extract:
DNA Fingerprinting – …
