Arrange the given steps involved in experiment conducted by Hershey–C…

The correct answer is Option (3) → (D), (C), (A), (B)

(D) They grew some viruses on medium containing radioactive phosphorus and some on radioactive sulfur — first. Phosphorus labels the viral DNA and sulfur labels the viral protein, so the two parts of the virus can be told apart later.

(C) Radioactive phages were allowed to attach to E. coli bacteria — second. The labelled viruses are needed before they can infect.

(A) The viral coats were removed from the bacteria by agitating them in a blender — third. This strips the empty viral coats off after infection.

(B) The virus particles were separated from the bacteria by spinning them in a centrifuge — last. The heavy bacteria settle and the light viral coats stay in the liquid, so the radioactivity of each can be checked.

NCERT Extract:
The Genetic Material Is DNA – “… Hershey and Chase worked to discover whether it was protein or DNA from the viruses that entered the bacteria. They grew some viruses on a medium that contained radioactive phosphorus and some others on medium that contained radioactive sulfur. Viruses grown in the presence of radioactive phosphorus contained radioactive DNA but not radioactive protein because DNA contains phosphorus but protein does not. Similarly, viruses grown on radioactive sulfur contained radioactive protein but not radioactive DNA because DNA does not contain sulfur. Radioactive phages were allowed to attach to E. coli bacteria. Then, as the infection proceeded, the viral coats were removed from the bacteria by agitating them in a blender. The virus particles were separated from the bacteria by spinning them in a centrifuge.”