Ex situ conservation of organisms does not include:

The correct answer is Option (4) → Sacred groves

Ex situ conservation protects threatened plants and animals outside their natural habitat. In situ conservation protects them within their natural habitat.

(1) Zoological parks — A method of ex situ conservation. Threatened animals are taken out of the wild and kept under special care.

(2) Wildlife safari — A method of ex situ conservation. Wildlife safari parks serve the same purpose as zoological parks and botanical gardens.

(3) Cryopreservation — A method of ex situ conservation. Gametes of threatened species are kept in a viable and fertile state for long periods at very low temperature.

(4) Sacred groves — Not a method of ex situ conservation. These are tracts of forest set aside and protected by cultural traditions. The plants and animals stay in their own habitat, so this is in situ conservation.

NCERT Extract:
How Do We Conserve Biodiversity? – “… Ex situ Conservation– In this approach, threatened animals and plants are taken out from their natural habitat and placed in special setting where they can be protected and given special care. Zoological parks, botanical gardens and wildlife safari parks serve this purpose. There are many animals that have become extinct in the wild but continue to be maintained in zoological parks. In recent years ex situ conservation has advanced beyond keeping threatened species in enclosures. Now gametes of threatened species can be preserved in viable and fertile condition for long periods using cryopreservation techniques, eggs can be fertilised in vitro, and plants can be propagated using tissue culture methods.”
How Do We Conserve Biodiversity? – “… In many cultures, tracts of forest were set aside, and all the trees and wildlife within were venerated and given total protection.”