Arrange the following steps of the process of recombinant DNA technology in correct order.
(A) Joining of foreign DNA to plasmid
(B) Isolation and cutting of DNA at specific locations
(C) Obtaining the foreign gene product
(D) Insertion of rDNA or ligated DNA into the host cell/organism
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 3
The correct answer is Option (3) → (B), (A), (D), (C)
(B) Isolation and cutting of DNA at specific locations — first. The DNA is first taken out of the cell in pure form. Restriction enzymes then cut it at specific sequences to release the gene of interest.
(A) Joining of foreign DNA to plasmid — second. The plasmid is cut with the same restriction enzyme, so its ends match those of the gene. DNA ligase joins the two, and recombinant DNA is formed.
(D) Insertion of rDNA or ligated DNA into the host cell/organism — third. The recombinant DNA is put into a competent host cell, such as E. coli, where it multiplies.
(C) Obtaining the foreign gene product — last. The host cells express the foreign gene and make its protein. They are grown on a large scale, and the product is then separated and purified.
NCERT Extract:
Processes of Recombinant DNA Technology – …
