Match List – I with List – II.

Choose the correct answer from the options given below:

(A)-(III), (B)-(I), (C)-(II), (D)-(V)

The correct answer is Option (2) → (A)-(III), (B)-(I), (C)-(II), (D)-(V)

A. Bt toxin is produced by a bacterium called Bacillus thuringiensis (Bt for short). Bt toxin gene has been cloned from the bacteria and been expressed in plants to provide resistance to insects without the need for insecticides; in effect created a bio-pesticide. B. thuringiensis forms protein crystals during a particular phase of their growth. These crystals contain a toxic insecticidal protein.

B. Several nematodes parasitise a wide variety of plants and animals including human beings. A nematode Meloidegyne incognitia infects the roots of tobacco plants and causes a great reduction in yield. A novel strategy was adopted to prevent this infestation which was based on the process of RNA interference (RNAi).

C. A nematode Meloidegyne incognitia infects the roots of tobacco plants which reduce the production of tobacco plants and reduces the production of tobacco. Thus, to prevent this infection a strategy is adopted known as RNA interface (RNAi) which takes place in all eukaryotic organisms as a method of cellular defence. By using Agrobacterium vectors, nematode-specific genes were introduced into the host plants which produce both sense and antisense RNA in the host cells. These two RNAs are complementary to each other and form a double stranded RNA (ds RNA) that initiates RNAi and hence, silences the specific mRNA of the nematode. 

D. In 1983, Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin.