Which of the following pairs is incorrectly matched? (A) Lysozyme Bac…

The correct answer is Option (2) → (B)

To isolate DNA, the cell must first be broken open. The book names an enzyme for each kind of cell, chosen by what its wall is made of: lysozyme for bacteria, cellulase for plant cells and chitinase for fungi. Restriction enzymes, which cut DNA at specific sites, are called molecular scissors.

Option (1) (A) Lysozyme – Bacteria — Correctly matched. Lysozyme breaks down the bacterial cell wall and is used to open bacterial cells.

Option (2) (B) Cellulase – Animal cell — Incorrectly matched. Cellulase digests cellulose, the material of the plant cell wall, so it is used for plant cells. Animal cells have no cellulose wall.

Option (3) (C) Restriction endonuclease – Molecular scissors — Correctly matched. Restriction endonucleases cut DNA at specific recognition sequences, and so they are called molecular scissors.

Option (4) (D) Chitinase – Fungus — Correctly matched. The fungal cell wall contains chitin, which chitinase breaks down.

NCERT Extract:
Isolation of the Genetic Material (DNA) – “… Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).”
Principles of Biotechnology – “… The cutting of DNA at specific locations became possible with the discovery of the so-called ‘molecular scissors’– restriction enzymes.”