In a continuous culture system :

Both the used medium is drained out, and high biomass production is achieved.

In a continuous culture system, cells are grown in a bioreactor where the used medium containing the cells is continuously or periodically drained out from one side. At the same time, fresh medium is continuously added from the other side to replace the drained medium. This ensures that the cells are maintained in their physiologically most active log/exponential phase, where they are actively dividing and growing.

The continuous culture system allows for a steady supply of nutrients and removal of waste products, creating an optimal environment for cell growth and protein production. As a result, the cells can continuously multiply and produce a larger biomass compared to batch culture methods, where the growth eventually slows down due to the depletion of nutrients and accumulation of waste products.

The higher biomass production achieved in the continuous culture system leads to higher yields of the desired protein. This is beneficial for large-scale production of recombinant proteins, where a significant amount of the protein is needed for various applications, such as research, therapeutics, or industrial processes. Additionally, continuous culture systems provide better control over the culture conditions, leading to more consistent and reproducible protein production.