Practicing Success
Practicing Success
CUET Preparation Today
What is elution ? |
Bright orange coloured bands of DNA in a ethidium bromide stained gel exposed to UV light . The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. It is a procedure through which a piece of DNA is introduced in a host bacterium. It is a way to introduce alien DNA into host cells. Condition in which any protein encoding gene is expressed in a heterologous host, |
Bright orange coloured bands of DNA in a ethidium bromide stained gel exposed to UV light . The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. |
During gel electrophoresis ,the separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation (you cannot see pure DNA fragments in the visible light and without staining). You can see bright orange coloured bands of DNA in a ethidium bromide stained gel exposed to UV light . The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.
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