Read the passage carefully and answer the given questions.

Recombinant DNA Technology

Asexual reproduction preserves the genetic information, while sexual reproduction permits variation. Traditional hybridization procedures used in plants and animals breeding very often lead to inclusion and multiplication of undesirable genes along with the desired genes. The techniques of genetic engineering, which include creation of recombinant DNA, use of gene cloning and gene transfer, overcome this limitation and allow us to isolate and introduce only one or a set of desirable genes without introducing genes into the target organism.

Which one of the following options is correct for working of restriction enzymes?

Cuts DNA at specific sequence

The correct answer is Option (4) → Cuts DNA at specific sequence

The cutting of DNA at specific locations became possible with the discovery of the so-called ‘molecular scissors’– restriction enzymes. Restriction enzymes belong to a larger class of enzymes called nucleases. These are of two kinds :exonuclease and endonuclease . Exonuclease remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA.