Read the following passage carefully and answer the given questions.

Insulin consists of two short polypeptide chains- chain A and chain B, that are linked together by disulphide bridges. Some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans, coleopterans and dipterans. Bt toxin protein is converted into an active form of toxin due to the alkaline pH of the gut which solubilise the crystals. Transgenic animals that produce useful biological products can be created by the introduction of the portion of DNA (or genes) which codes for a particular product such as human protein (α-1-antitrypsin) used to treat emphysema. Plants, bacteria, fungi and animals whose genes have been altered by manipulation are called Genetically Modified Organisms (GMO). GM plants have been useful in many ways. Genetic modification has: (i) made crops more tolerant to abiotic stresses. (ii) reduced reliance on chemical pesticides (pest-resistant crops). (iii) helped to reduce post harvest losses. (iv) increased efficiency of mineral usage by plants (this prevents early exhaustion of fertility of soil). (v) enhanced nutritional value of food.

The beetles that infest Bt cotton plant are killed:

Due to conversion of protoxins into toxin in alkaline pH of the gut of the insects that causes lysis of midgut epithelial cells.

The correct answer is Option (2) → Due to conversion of protoxins into toxin in alkaline pH of the gut of the insects that causes lysis of midgut epithelial cells.

Bt toxin is produced by a bacterium called Bacillus thuringiensis (Bt for short). Bt toxin gene has been cloned from the bacteria and been expressed in plants to provide resistance to insects without the need for insecticides; in effect created a bio-pesticide. Examples are Bt cotton, Bt corn, rice, tomato, potato and soyabean etc.

Bt Cotton: Some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans (tobacco budworm, armyworm), coleopterans (beetles) and dipterans (flies, mosquitoes). B. thuringiensis forms protein crystals during a particular phase of their growth. These crystals contain a toxic insecticidal protein. Actually, the Bt toxin protein exist as inactive protoxins but once an insect ingest the inactive toxin, it is converted into an active form of toxin due to the alkaline pH of the gut which solubilise the crystals. The activated toxin binds to the surface of midgut epithelial cells and create pores that cause cell swelling and lysis and eventually cause death of the insect. The toxin is coded by a gene cryIAc named cry. There are a number of them, for example, the proteins encoded by the genes cryIAc and cryIIAb control the cotton bollworms, that of cryIAb controls corn borer.