Steps of preparation of human insulin are given below Arrange these steps in a proper sequence
A. Chain 'A' and 'B' are extracted separately.
B. Chain A and B are joined to plasmids of different E. coli for replication
C. Preparation of two chains of DNA corresponding to chain 'A' and 'B' of human insulin
D. Chain 'A' and 'B' are joined to each other by disulphide bond
E. Mature insulin is obtained
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 1
The correct answer is Option (1) → C, B, A, D, E
Two DNA sequences for chains A and B are prepared (C), joined to plasmids of E. coli (B), the chains are produced and extracted separately (A), joined by disulphide bonds (D), and mature insulin is obtained (E).
NCERT Extract:
Genetically Engineered Insulin – “… In 1983, Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin.”