Match List-I with List-II:

Choose the correct answer from the options given below:

(A)-(III), (B)-(IV), (C)-(I), (D)-(II)

The correct answer is Option (2) → (A)-(III), (B)-(IV), (C)-(I), (D)-(II)

A. Insulin is composed of two short polypeptide chains, namely chain A and chain B, which are connected by disulfide bridges. In mammals, including humans, insulin is initially produced as a prohormone, similar to a proenzyme that requires processing to become a fully mature and functional hormone. This prohormone contains an additional segment called the C-peptide. However, during the maturation process into insulin, the C-peptide is removed, and it is not present in the mature insulin.

B. Transgenic animals that produce useful biological products can be created by the introduction of the portion of DNA (or genes) which codes for a particular product such as human protein (α-1-antitrypsin) used to treat emphysema.

C. The toxin is coded by a gene cryIAc named cry. There are a number of them, for example, the proteins encoded by the genes cryIAc and cryIIAb control the cotton bollworms, that of cryIAb controls corn borer.

D. In 1997, scientists achieved a significant breakthrough by creating the first transgenic cow named Rosie. This cow was genetically modified to produce human protein-enriched milk, specifically containing human alpha-lactalbumin. Alpha-lactalbumin is a protein found in human breast milk that plays a crucial role in providing essential nutrients and immune factors to human infants.