Practicing Success
Assertion: Genetic engineering requires both nucleases and ligases. |
Both Assertion (A) and Reason (R) are correct and R is the correct explanation of A. Both Assertion (A) and Reason (R) are correct but R is not the correct explanation of A. Assertion (A) is true but Reason(R) is not correct. Both Assertion (A) and Reasoning are false (F ). |
Assertion (A) is true but Reason(R) is not correct. |
Nucleases are the enzymes that remove nucleotides or produce nick in the DNA strand. Exonucleases remove nucleotides from the free ends of DNA while endonucleases produce internal nick in DNA. Now, the desired gene is inserted and the cut ends are sealed with the help of DNA ligase. Ligases are also called molecular glue as they join together two strands by forming phosphodiester bonds between adjacent nucleotides. |