Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Assertion: Genetic engineering requires both nucleases and ligases.
Reason: Ligases produce the nick in the recombinant DNA molecule.

Options:

Both Assertion (A) and Reason (R) are correct and R is the correct explanation of A.

Both Assertion (A) and Reason (R) are correct but R is not the correct explanation of A.

Assertion (A) is true but Reason(R) is not correct.

Both Assertion (A) and Reasoning are false (F ).

Correct Answer:

Assertion (A) is true but Reason(R) is not correct.

Explanation:

Nucleases are the enzymes that remove nucleotides or produce nick in the DNA strand. Exonucleases remove nucleotides from the free ends of DNA while endonucleases produce internal nick in DNA. Now, the desired gene is inserted and the cut ends are sealed with the help of DNA ligase. Ligases are also called molecular glue as they join together two strands by forming phosphodiester bonds between adjacent nucleotides.