Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

In biolistic method, the cells are bombarded with high velocity microparticles of:

Options:

DNA coated with gold or tungsten

Gold or tungsten coated with DNA

Platinum or copper coated with DNA

Copper or iron coated with DNA

Correct Answer:

Gold or tungsten coated with DNA

Explanation:

The correct answer is Option (2) → Gold or tungsten coated with DNA

There are several methods to introduce alien DNA into host cells. One such method is micro-injection, where recombinant DNA is directly injected into the nucleus of an animal cell.

Biolistics: Biolistics is a method of introducing foreign DNA into host cells, particularly plant cells, using high-velocity micro particles. In this technique, small particles, such as gold or tungsten, are coated with the desired DNA and then shot into the target cells using a device known as a gene gun. The high velocity of the particles allows them to penetrate the cell wall and deliver the DNA into the host cell's nucleus.