Determine the correct order with respect to genetic modification of an organism: 1. Linking of target gene with the plasmid vector 2. Transfer of recombinant DNA to bacteria 3. Replication of recombinant DNA using the host's DNA polymerase 4. cutting of DNA at specific locations to isolate the target gene

1,2,3,4 4,1,3,2 4,1,2,3 2,3,4,1

4,1,2,3

Genetic modification of an organism includes the steps in following order: 1. the cutting of DNA at specific locations with molecular scissors: restriction enzymes 2. the cut piece are then linked with plasmid DNA to form a recombinant DNA using DNA Ligases. 3. DNA is then transferred to a bacterium. 4. The recombinant DNA can now replicate using host's DNA polymerase enzyme and make multiple copies. the ability of making multiple copies is called cloning.