Target Exam

CUET

Subject

Biology

Chapter

Molecular Basis of Inheritance

Question:

Match List I with List II.

List I(Name of Scientists)  List II (Discovery)
A. Alec Jeffreys I. Lac Operon
B. Fancois Jacob and Jacque Monod II. Deciphering of genetic code 
C. Marshall Nirenberg  III. Semiconservative replication of DNA 
D. Masselson Stahl IV. DNA Fingerprinting 

Choose the correct answer from the options given below:

Options:

A-IV. B-I, C-II. D-III

A-I.B III. C-IV. D-II 

A-II.B III. C-I, D-IV 

A-IV. B-II. C-III. D-I 

Correct Answer:

A-IV. B-I, C-II. D-III

Explanation:

The correct answer is Option (1) -A-IV. B-I, C-II. D-III

List I (Name of Scientists)  List II (Discovery)
A. Alec Jeffreys IV. DNA Fingerprinting
B. Fancois Jacob and Jacque Monod I. Lac Operon
C. Marshall Nirenberg  II. Deciphering of genetic code
D. Masselson Stahl III. Semiconservative replication of DNA

A. The technique of DNA Fingerprinting was initially developed by Alec Jeffreys. He used a satellite DNA as probe that shows very high degree of polymorphism. It was called as Variable Number of Tandem Repeats (VNTR). The technique, as used earlier, involved Southern blot hybridisation using radiolabelled VNTR as a probe.

B. The lac operon is an operon responsible for transport and metabolism of lactose in Escherichia coli and other bacteria. The mechanisms of regulation of expression of genes responsible for coding the involved enzymes was first explained by Francois Jacob and Jacque Monad. They were the first to elucidate a transcriptionally regulated system. In lac operon (here lac refers to lactose), a polycistronic structural gene is regulated by a common promoter and regulatory genes. Such arrangement is very common in bacteria and is referred to as operon.

C. Marshall Nirenberg’s cell-free system for protein synthesis helped the code to be deciphered.

D. Meselson and Stahl Experiment was an experimental proof for semiconservative DNA replication. In 1958, Matthew Meselson and Franklin Stahl conducted an experiment on E. coli which divides in 20 minutes, to study the replication of DNA.