- a-Smaller pore size.
- b-The tighter network of agarose fibers.
- c-The increased electrostatic interactions between the negatively charged DNA fragments and the positively charged gel matrix .
- d-All of these is the correct option .
- While it is true that high-percentage agarose gels have smaller pore sizes, it is not the main reason why large DNA fragments tend to accumulate near the wells.
- The pore size of the agarose gel is determined by the percentage of agarose used in the gel. A higher percentage of agarose will result in a smaller pore size, which can affect the mobility of DNA fragments of different sizes. Small DNA fragments can move more easily through the smaller pores, while larger fragments can get stuck or move more slowly.
- However, the main reason why large DNA fragments tend to accumulate near the wells is that they experience a higher degree of resistance to movement through the gel. This is due to a combination of factors, including the smaller pore size, the tighter network of agarose fibers, and the increased electrostatic interactions between the negatively charged DNA fragments and the positively charged gel matrix near the wells.
- Therefore, while the pore size of the agarose gel can contribute to the accumulation of large DNA fragments near the wells, it is not the only or the primary factor. The tight network of agarose fibers and the electrostatic interactions play a more significant role in impeding the movement of large DNA fragments through the gel.
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