Arrange the following processes of recombinant DNA technology in a correct sequence:
(A). Amplification of gene by using PCR
(B). Isolation of DNA and cutting of DNA at specific location
(C). Obtaining the desired foreign gene product
(D). Insertion of recombinant DNA into the host
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 3
The correct answer is Option (3) → (B), (A), (D), (C)
(B) Isolation of DNA and cutting of DNA at specific location — first. The DNA must first be taken out of the cell in pure form. Restriction enzymes then cut it at specific recognition sequences to release the gene of interest.
(A) Amplification of gene by using PCR — second. The cut gene of interest is copied many times by PCR, so that enough of it is available to join to vectors.
(D) Insertion of recombinant DNA into the host — third. The gene is ligated into a vector to form recombinant DNA, which is then put into a competent host cell, such as E. coli.
(C) Obtaining the desired foreign gene product — last. The host cells express the foreign gene and make its protein. They are grown on a large scale in bioreactors, and the product is extracted and purified.
NCERT Extract:
Processes of Recombinant DNA Technology – …
