The enzyme which is used to remove nucleotides from the ends of DNA fragment is-

Restriction exonuclease

The correct answer is Option (3) → Restriction exonuclease

The cutting of DNA at specific locations became possible with the discovery of the so-called ‘molecular scissors’– restriction enzymes. Restriction enzymes belong to a larger class of enzymes called nucleases. These are of two kinds; exonucleases and endonucleases. Exonucleases remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA.