Practicing Success
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| Which was the main challenge for production of insulin using rDNA techniques ? |
Removal of C peptide from mature insulin . Getting insulin assembled into a mature form . Synthesis of insulin as a pro-hormone. None of these. |
| Getting insulin assembled into a mature form . |
| The main challenge for production of insulin using rDNA techniques was getting insulin assembled into a mature form. In 1983, Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin. |
