Which was the main challenge for production of insulin using rDNA techniques ?
Answer & explanation
Correct answer: option 2
The main
challenge for production of insulin using rDNA techniques
was getting insulin assembled into a mature form. In
1983, Eli Lilly an American company prepared two DNA sequences
corresponding to A and B, chains of human insulin and introduced them
in plasmids of E. coli to produce insulin chains. Chains A and B were
produced separately, extracted and combined by creating disulfide bonds
to form human insulin.