Which among the following microbes are mostly used in genetic engineering technology ?

Bacillus thuringiensis and Streptococcus. Staphylococcus and Vibrio cholerae . Escherichia coli and Agrobacterium tumefaciens . None of these .

Escherichia coli and Agrobacterium tumefaciens .

Genetic engineering is a branch of science that uses various biotechnological techniques to manipulate an organism's genome to create species with desired traits. It has been found that of these, E.coli has the ability to transfer DNA laterally to and from other species .The tumor inducing (Ti) plasmid of Agrobacterium tumifaciens has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants.